Spinning off by the favorite Professor Layton puzzle games, that this picture brings Layton along with his trusty assistant Luke into the big screen allowing fans to enjoy more of the very same. Layton receives a letter by the opera singer inviting them to watch her performance. She also asks Layton, Luke, and Remi for assistance in a puzzle, saying her friend Melina went lost 12 months past but came back as a seven year-old girl had found the secret of eternal life. Of course, Layton along with his apprentices fascinates, therefore they go towards the Crown Petone opera house. When they arrive, something is very strange: the pianist, Ozro Vislar speaks about a game, and the winner will win the"eternal existence " Until it is too late it is Around Layton, Luke, and Remi to solve the puzzle of their Eternal Diva.